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Identifying corneal infections in formalin-fixed specimens ...- are formalin fixed tissues infectious ,METHODS. This retrospective study evaluated 16 cases of infectious keratitis and four control corneas in formalin-fixed tissues from the pathology laboratory. Infectious cases also were analyzed in the microbiology laboratory using culture, polymerase chain reaction, and direct staining.Identifying corneal infections in formalin-fixed specimens ...Purpose: We test the ability of next-generation sequencing, combined with computational analysis, to identify a range of organisms causing infectious keratitis. Methods: This retrospective study evaluated 16 cases of infectious keratitis and four control corneas in formalin-fixed tissues from the pathology laboratory. Infectious cases also were analyzed in the microbiology laboratory using...



Identifying corneal infections in formalin-fixed specimens ...

Purpose: We test the ability of next-generation sequencing, combined with computational analysis, to identify a range of organisms causing infectious keratitis. Methods: This retrospective study evaluated 16 cases of infectious keratitis and four control corneas in formalin-fixed tissues from the pathology laboratory. Infectious cases also were analyzed in the microbiology laboratory using...

Effects of prolonged formalin fixation on the ...

Effects of prolonged formalin fixation on the immunohistochemical detection of infectious agents in formalin-fixed, paraffin-embedded tissues. Vet Pathol. 2010; 47(3):529-35 (ISSN: 1544-2217)

(PDF) The mechanical properties of fresh versus fresh ...

Wilke reported an 80% decrease in range of motion for formalin fixed spinal specimens, confirming cadavers are not truly representative of in vivo conditions [Wilke et al., 1996]. Soft- fix embalming techniques have the benefit of potentially reduced health risks while also maintaining the physical properties of cadavers so they remain more ...

Detection of infectious laryngotracheitis virus in ...

Detection of infectious laryngotracheitis virus in formalin-fixed, paraffin-embedded tissues by nested polymerase chain reaction. Abstract Infectious laryngotracheitis virus (ILTV) is routinely diagnosed by histopathologic examination of trachea, eyelid and lung tissues.

Detection of infectious laryngotracheitis virus in ...

To further validate the ability of this assay to detect ILT, nested PCR was performed in formalin-fixed, paraffin-embedded tissues from 35 cases of respiratory disease. Of the 35 cases, 12 were considered ILT suspects on the basis of initial clinical observation.

Enhanced phylogenetic resolution of Newcastle disease ...

May 14, 2019·In contrast, formalin fixation inactivates infectious agents; thus, formalin-fixed paraffin-embedded (FFPE) tissues can be easily, safely, and affordably transported across boundaries and processed in a biosafety level 1 laboratory . Additionally, formalin fixation is the gold standard for pathologic preservation and FFPE tissues allow for a ...

Improving the Proteomic Analysis of Formalin-Fixed Tissue

Jul 06, 2021·A potential solution to this dilemma is the millions of archived formalin-fixed, paraffin-embedded (FFPE) tissues for which the clinical course of disease, response to …

Formalin RT-QuIC assay detects prion-seeding activity in ...

Sep 10, 2021·Results. The SD 50 values of formalin-fixed brain samples from 14 MM1 cases, 2 MM2C cases, and 2 MM2T cases were 10 7.77±0.57 /g tissue, 10 7.44±0.24 /g tissue and 10 6.00±0.77 /g tissue, respectively. The average SD 50 value in MM1 unfixed brains decreased by 10 2.04 after formalin fixation for 1 month. In MM1 cases, after combined formalin and formic acid treatment, the …

Can we perform TB culture and PCR on formalinized tissue ...

Jan 01, 2013·Of the 60 formalin-fixed, paraffin-embedded tissue specimens studied, 57 showed granulomatous inflammation and 53 had been cultured for mycobacteria; 10 were positive for M. tuberculosis and three ...

Effects of Prolonged Formalin Fixation on the ...

Mar 23, 2010·Therefore, the goal of this study was to evaluate the effects of prolonged formalin fixation on the immunohistochemical detection of 21 infectious agents. Tissue slices about 5 mm thick were fixed in 10% neutral-buffered formalin, processed, and paraffin embedded at day 1 or 2 and then at approximately weekly intervals.

Can we perform TB culture and PCR on formalinized tissue ...

Jan 01, 2013·Of the 60 formalin-fixed, paraffin-embedded tissue specimens studied, 57 showed granulomatous inflammation and 53 had been cultured for mycobacteria; 10 were positive for M. tuberculosis and three ...

Identifying corneal infections in formalin-fixed specimens ...

Purpose: We test the ability of next-generation sequencing, combined with computational analysis, to identify a range of organisms causing infectious keratitis. Methods: This retrospective study evaluated 16 cases of infectious keratitis and four control corneas in formalin-fixed tissues from the pathology laboratory. Infectious cases also were analyzed in the microbiology laboratory using...

Detection of infectious bursal disease virus from formalin ...

Formalin fixed paraffin embedded tissues blocks are used routinely to diagnose the economically important immunosuppressive infectious bursal disease virus (IBDV) in chickens. Immunohistochemical detection of viruses in tissue blocks has been done with varying results between laboratories.

Detection of diatom in formalin-fixed tissue by proteinase ...

Sep 10, 2009·The results of this study suggest that diatom detection with proteinase K digestion can be implemented in formalin-fixed tissue by heating the samples in the glycine-containing buffer. It is recommended to use formalin-fixed tissues than unfixed tissues for the diatom test in cases of bodies afflicted with highly infectious disease.

Detection of Infectious Laryngotracheitis Virus in ...

Jan 01, 2002·Jennifer Humberd, Maricarmen García, Sylva M. Riblet, R. S. Resurreccion, and T. P. Brown "Detection of Infectious Laryngotracheitis Virus in Formalin-Fixed, Paraffin-Embedded Tissues by Nested Polymerase Chain Reaction," Avian Diseases 46(1), 64-74, (1 January 2002).

Detection of infectious laryngotracheitis virus in ...

To further validate the ability of this assay to detect ILT, nested PCR was performed in formalin-fixed, paraffin-embedded tissues from 35 cases of respiratory …

Detection of Infectious Laryngotracheitis Virus in ...

Jan 01, 2002·Jennifer Humberd, Maricarmen García, Sylva M. Riblet, R. S. Resurreccion, and T. P. Brown "Detection of Infectious Laryngotracheitis Virus in Formalin-Fixed, Paraffin-Embedded Tissues by Nested Polymerase Chain Reaction," Avian Diseases 46(1), 64-74, (1 January 2002).

Identifying corneal infections in formalin-fixed specimens ...

Purpose: We test the ability of next-generation sequencing, combined with computational analysis, to identify a range of organisms causing infectious keratitis. Methods: This retrospective study evaluated 16 cases of infectious keratitis and four control corneas in formalin-fixed tissues from the pathology laboratory. Infectious cases also were analyzed in the microbiology laboratory using...

(PDF) TaqMan® real-time RT-PCR detection of infectious ...

TaqMan® real-time RT-PCR detection of infectious salmon anaemia virus (ISAV) from formalin-fixed paraffin-embedded Atlantic salmon Salmo salar tissues. Diseases of Aquatic Organisms, 2010. Marcos Godoy. Download PDF. Download Full PDF Package.

Difficulties in Differentiating Coronaviruses from ...

Feb 18, 2021·Although formalin- or glutaraldehyde-fixed tissues embedded for EM are necessary for accurate classification of viral morphology and morphogenesis, the detection of virus within a tissue may require a more targeted approach to find the infected area, such as by working closely with a pathologist to select promising areas for EM from formalin ...

Effects of Prolonged Formalin Fixation on the ...

Mar 23, 2010·Therefore, the goal of this study was to evaluate the effects of prolonged formalin fixation on the immunohistochemical detection of 21 infectious agents. Tissue slices about 5 mm thick were fixed in 10% neutral-buffered formalin, processed, and paraffin embedded at day 1 or 2 and then at approximately weekly intervals.

Policy Statement: Select agent contained in formalin-fixed ...

Oct 09, 2018·FSAP has determined that a select agent or regulated nucleic acids that can produce infectious forms of any select agent virus contained in a formalin-fixed paraffin-embedded (FFPE) tissue has been effectively inactivated if the FFPE process used is a recognized method (e.g. a previously published method shown to be effective such that ...

Formalin RT-QuIC assay detects prion-seeding activity in ...

Sep 10, 2021·Results. The SD 50 values of formalin-fixed brain samples from 14 MM1 cases, 2 MM2C cases, and 2 MM2T cases were 10 7.77±0.57 /g tissue, 10 7.44±0.24 /g tissue and 10 6.00±0.77 /g tissue, respectively. The average SD 50 value in MM1 unfixed brains decreased by 10 2.04 after formalin fixation for 1 month. In MM1 cases, after combined formalin and formic acid treatment, the …

Immunohistochemistry - WADDL

Infectious Agents . Tissues in 10% Formalin OR Formalin fixed paraffin-embedded tissue. Leakproof jar or Styrofoam mailer: 7 working days. Results interpreted as “Detected” or “Not Detected” and reported separately from pathology report. Tumor Markers: Tissues in 10% Formalin OR Formalin fixed paraffin-embedded tissue. Leakproof jar or ...

USDA APHIS | Packaging and Labeling Submissions to the NVSL

Jun 02, 2020·Pack fresh and formalin-fixed specimens separately. Ship in separate containers when possible. Double bag all fresh tissues. Formalin-fixed specimens: Specimens may be shipped in a plastic specimen jar containing a 10:1 (liquid to tissue) volume of formalin. Do NOT use bags (Whirlpacks, zipper-closure, or heat-sealed) in lieu of a jar.

Difficulties in Differentiating Coronaviruses from ...

Feb 18, 2021·Although formalin- or glutaraldehyde-fixed tissues embedded for EM are necessary for accurate classification of viral morphology and morphogenesis, the detection of virus within a tissue may require a more targeted approach to find the infected area, such as by working closely with a pathologist to select promising areas for EM from formalin ...